Related Experiment Video
Updated: Jun 28, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Spectrophotometric determination of nitrite based on its catalytic effect on the oxidation of carminic acid by
J L Manzoori1, M H Sorouraddin, A M Haji-Shabani
1Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Abstract:
A highly sensitive and selective method is described for the determination of trace amounts of nitrite based on its effect on the oxidation of carminic acid with bromate. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of carminic acid at 490 nm after 3 min of mixing the reagents. The optimum reaction conditions were 1.8x10(-1) mol l(-1) H(2)SO(4), 3.8x10(-3) mol l(-1) KBrO(3), and 1.2x10(-4) mol l(-1) carminic acid at 30 degrees C. By using the recommended procedure, the calibration graph was linear from 0.2 to 14 ng ml(-1) of nitrite; the detection limit was 0.04 ng ml(-1); the R.S.D. for six replicate determinations of 6 ng ml(-1) was 1.7%. The method is mostly free from interference, especially from large amounts of nitrate and ammonium ions. The proposed method was applied to the determination of nitrite in rain and river water.
Related Concept Videos
Rate-Determining Steps
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
Electrophilic Aromatic Substitution: Nitration of Benzene
2° Amines to N-Nitrosamines: Reaction with NaNO2
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by water loss...

