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Updated: Jun 28, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Flow-injection catalytic spectrophotometric determination of nitrite using the redox reaction between naphthol green
Z Zhi-Qi1, G Lou-Jun, Z Han-Ying
1Central Laboratory, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Abstract:
A flow injection catalytic spectrophotometric method is proposed for the determination of nitrite based on its catalytic effect on the redox reaction between naphthol green B and potassium bromate in phosphoric acid medium. The reaction is monitored spectrophotometrically by measuring the decrease in absorbance of naphthol green B at the maximum absorption wavelength of 722 nm. The detection limit is 0.5 ng cm(-3) in a sample volume of 90 mm(3). Up to 50 samples can be analyzed per hour with a relative precision of ca. 2%. The method is free from most interferences, especially from large amounts of nitrate. The procedure was successfully applied to the determination of trace nitrite in natural waters.
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