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DNA Stable-Isotope Probing (DNA-SIP)
Published on: August 2, 2010
Effect of temperature on DNA fractionation in slalom chromatography
E Peyrin1, Y C Guillaume, C Garrel
1Laboratoire de Chimie Analytique, UFR de Pharmacie, Domaine de la Merci, 38700 La Tronche, France.
Abstract:
Slalom chromatography is an alternative chromatographic procedure for the analysis of relatively large double-stranded DNA molecules and is based on a hydrodynamic principle. The retardation of the DNA fragments from the cleavage of the Lambda DNA by the KpnI restriction enzyme was studied using an acetonitrile-phosphate buffer as a mobile phase (flow rate equal to 0.3 ml/min) and a C1 column as a stationary phase at various temperatures. It was shown that the temperature constituted an important parameter for the separation of the DNA fragments in slalom chromatography. The DNA hydrodynamic behavior with the temperature was related to the variation in the fluid viscosity and the modification of the elastic properties of the biopolyrner.
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