Chemiluminescence sensor for isoniazid with controlled-reagent-release technology

Area of Science:

• Analytical Chemistry
• Chemical Sensing

Background:

• Isoniazid is a crucial anti-tuberculosis drug.
• Accurate quantification of isoniazid is essential for pharmaceutical quality control.
• Existing analytical methods may lack speed, sensitivity, or reusability.

Purpose of the Study:

• To develop a novel chemiluminescence (CL) sensor for the sensitive determination of isoniazid.
• To integrate the CL sensor with flow-injection technology for automated analysis.
• To evaluate the sensor's performance and applicability in pharmaceutical samples.

Main Methods:

• Immobilization of luminol and ferricyanide on an anion-exchange column.
• Utilizing flow-injection analysis for reagent elution and signal generation.
• Measuring the decrease in CL intensity caused by isoniazid.
• Determining linearity, detection limit, and precision.

Main Results:

• The chemiluminescence signal decreased linearly with isoniazid concentration from 0.001-1.0 µg/mL.
• A low detection limit of 0.35 ng/mL (3s) was achieved.
• The entire analytical process, including sampling and washing, was completed within 2 minutes.
• The sensor demonstrated excellent reusability, with over 400 cycles, and a relative standard deviation below 4.1%.

Conclusions:

• A novel, highly sensitive, and reusable chemiluminescence sensor for isoniazid has been successfully developed.
• The integrated flow-injection system provides a rapid and efficient method for isoniazid determination.
• The sensor is suitable for the accurate quantification of isoniazid in pharmaceutical preparations.