Creatinine determination in urine samples by batchwise kinetic procedure and flow injection analysis using the Jaffé...

Area of Science:

• Clinical Chemistry
• Analytical Chemistry
• Biochemistry

Background:

• The Jaffé reaction is a classical method for determining creatinine in urine.
• Accurate creatinine measurement is crucial for diagnosing kidney function and other health conditions.
• Existing methods may suffer from bias and imprecision, necessitating analytical improvements.

Purpose of the Study:

• To compare the analytical characteristics of batchwise and flow injection (FI) methods for urine creatinine determination.
• To minimize bias error and enhance precision in creatinine measurements.
• To investigate the impact of albumin concentration on creatinine determination.

Main Methods:

• Comparative analysis of batchwise and FI methods based on the Jaffé reaction.
• Evaluation of analytical signals, including absorbance increments at different time points.
• Assessment of method performance with varying albumin concentrations in urine samples.

Main Results:

• The batchwise method allows bias-free urine creatinine estimation using calibration graphs without blank measurement, with a linear range of 0.92-50 mg/l and a sample rate of 7/hour.
• The FI method offers a higher sample rate (approx. 20/hour) with a linear range of 30-100 mg/l but requires albumin correction for accuracy.
• Accurate creatinine results were achieved with both methods, confirming their diagnostic utility.

Conclusions:

• The batchwise Jaffé reaction method provides accurate and precise urine creatinine measurements, suitable for clinical diagnostics.
• The FI method is efficient for high-throughput analysis but necessitates specific calibration strategies when albumin levels are elevated.
• Both optimized methods are valuable tools for establishing urinary creatinine levels and aiding in disease diagnosis.