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Updated: Jun 28, 2026

Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
Published on: August 22, 2017
Stopped-flow fluorimetric determination of amoxycillin and clavulanic acid by partial least-squares multivariate
A Muñoz de la Peña1, M I Acedo-Valenzuela, A Espinosa-Mansilla
1Department of Analytical Chemistry, Faculty of Sciences, University of Extremadura, Avda Elvas s/n 06071 Badajoz, Spain.
Abstract:
Mixtures of amoxycillin and clavulanic acid were determined by using kinetic data in combination with partial least-squares multivariate calibration. The reaction of oxidation of these compounds with cerium(IV) in sulfuric acid medium has been monitored fluorimetrically. To follow the kinetics of the reaction, the stopped-flow mixing technique was used. Partial least-squares calibration of the kinetic data allowed the resolution of the analytes investigated in the concentration ranges between 0 and 4 mugml(-1). The method was applied satisfactorily to several pharmaceutical formulations, including Clavucid, Augmentine, Pangamox, Eupeclanic and Clamoxyl. The results obtained were validated by using an HPLC method. The percentages of recovery range from 91 to 105% for amoxycillin and from 78 to 117% for clavulanic acid, respectively.
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