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Updated: Jun 28, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
The application of spline wavelet transform to the determination of electroosmotic mobility in capillary
Xiong Jianhui1, Xu Guowang, Zhang Pudun
1National Chromatography R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Science, ZhongShan Road 161, Dalian 116011, PR China.
Abstract:
The rule of current change was studied during capillary electrophoresis (CE) separation process while the conductivity of the sample solution was different from that of the buffer. Using a quadratic spline wavelet of compact support, the wavelet transforms (WTs) of capillary electrophoretic currents were performed. The time corresponding to the maximum of WT coefficients was chosen as the time of current inflection to calculate electroosmotic mobility. The proposed method was suitable for different CE modes, including capillary zone electrophoresis, nonaqueous CE and micellar electrokinetic chromatography. Compared with the neutral marker method, the relative errors of the developed method for the determination of electroosmotic mobility were all below 2.5%.
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