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Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
Published on: December 7, 2010
Immunosensor for the determination of azidothymidine: its utilization as detector in a sequential injection analysis
Raluca-Ioana Stefan1, Rahel Girmai Bokretsion, Jacobus Frederick van Staden
1Department of Chemistry, University of Pretoria, Pretoria 0002, South Africa. raluca.stefan@chem.up.ac.za
Talanta
|October 31, 2008
Summary
A novel amperometric immunosensor using graphite paste was developed for azidothymidine (AZT) detection. This reliable sensor is effective for pharmaceutical analysis and can be regenerated for repeated use.
Area of Science:
- Electrochemistry
- Biosensors
- Analytical Chemistry
Background:
- Azidothymidine (AZT) is a crucial antiviral medication.
- Accurate quantification of AZT in pharmaceutical formulations is essential for quality control.
Purpose of the Study:
- To develop and validate a cost-effective amperometric immunosensor for azidothymidine (AZT) detection.
- To assess the immunosensor's performance in pharmaceutical formulations and raw material analysis.
Main Methods:
- Construction of an amperometric immunosensor using anti-AZT-impregnated graphite paste.
- Electrochemical detection of AZT at a potential of 435 mV vs Ag/AgCl.
- Regeneration of the immunosensor surface through polishing.
- Integration of the immunosensor into a sequential injection analysis system.
Main Results:
- The immunosensor demonstrated reliable performance for AZT assay in pharmaceutical formulations.
- The sensor surface could be easily regenerated, allowing for multiple assays.
- Successful application as a detector in sequential injection analysis for on-line purity and content determination of AZT.
Conclusions:
- The developed graphite paste-based amperometric immunosensor offers a reliable and reusable platform for AZT quantification.
- This method is suitable for quality control of AZT in both raw materials and finished pharmaceutical products.
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