Related Experiment Video
Updated: Jun 28, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Determination of protonation- and metal complex stability constants for a chelating monomer and its immobilized in
1Department of Analytical Chemistry, University of Veszprém P.O. Box 158, H-8201 Veszprém, Hungary.
Abstract:
An improved method is described for the calculation of complex stability constants for metal-ligand complexes considering ligands immobilized in resin phase. The applicability of it has been proved for N'-benzylethylene diamine N,N,N'-triacetic acid monomer (BEDTA) and for the ion exchange resin developed in the authors laboratory immobilizing this with styrene divinyl benzene. Calcium and magnesium metal ion complexes were investigated. Electrochemical and flame photometric measurements were used to collect equilibrium concentration data. The procedure worked out included the measurement of the quantity of resin bound water. Using these and the other experimentally gathered values and the improved way of calculation metal ligand complex stability constants were determined in aqueous media. Ion exchange chromatographic separation of calcium and magnesium ions was performed with a resin containing column for separation and optimized eluent.
More Related Videos
11:04Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
Published on: September 7, 2019
10:27Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Related Concept Videos
Complexation Equilibria: The Chelate Effect
Complexation Equilibria: Factors Influencing Stability of Complexes
EDTA: Chemistry and Properties
Complexometric Titration: Ligands
Extraction: Advanced Methods
Metal-Ligand Bonds
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...