Fluorometric sensors based on chemically modified enzymes Glucose determination in drinks

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Biotechnology

Background:

• Accurate glucose determination is crucial for beverage quality control.
• Existing methods for glucose measurement may have limitations in speed or sample handling.
• Development of rapid and sensitive glucose sensors is an ongoing area of research.

Purpose of the Study:

• To develop and characterize an enzymatic fluorometric sensor for glucose determination in drinks.
• To optimize the sensor's performance for flow injection analysis (FIA).
• To evaluate the sensor's accuracy, reproducibility, and operational stability.

Main Methods:

• Immobilization of chemically modified glucose oxidase (GOx-FS) in a polyacrylamide polymer film.
• Utilizing changes in fluorescence intensity to quantify glucose concentration.
• Optimizing flow rate, sample injection volume, and pH for FIA mode.

Main Results:

• The sensor demonstrated a linear response to glucose concentrations from 400 to 2000 mg l(-1).
• Optimal FIA conditions included a flow rate of 0.7 ml min(-1), 300 μl sample injection, and pH 6.5.
• The sensor exhibited good reproducibility (around 3%) and a long operational lifetime (at least 3 months/350 measurements).

Conclusions:

• The enzymatic fluorometric sensor provides accurate glucose determination in drinks.
• The sensor design effectively mitigates interference from the filter effect.
• This sensor represents a viable tool for routine beverage analysis.