Simultaneous determination of creatine and creatinine using amperometric biosensors

Area of Science:

• Analytical Chemistry
• Biotechnology
• Electrochemistry

Background:

• Creatine and creatinine are important biomarkers in physiological and clinical diagnostics.
• Accurate and sensitive detection methods are crucial for their quantification.
• Amperometric biosensors offer a promising platform for rapid and selective analyte determination.

Purpose of the Study:

• To develop and validate amperometric biosensors for the determination of creatine and creatinine.
• To establish sensitive detection ranges and reliable performance metrics for the proposed biosensors.

Main Methods:

• A bienzymatic biosensor utilizing creatinase (CI) and sarcosine oxidase (SO) was employed for creatine detection.
• A trienzymatic biosensor incorporating CI, SO, and creatininase (CA) was developed for creatinine determination.
• Amperometric detection was utilized to quantify the enzymatic reaction products.

Main Results:

• The biosensors demonstrated linear concentration ranges from the pmol L⁻¹ to nmol L⁻¹ magnitude.
• Very low limits of detection were achieved for both creatine and creatinine.
• The biosensors exhibited high reliability in quantifying creatine and creatinine in raw materials and pharmaceutical formulations.

Conclusions:

• The developed amperometric biosensors provide a sensitive and reliable method for creatine and creatinine analysis.
• These biosensors are suitable for quality control applications in pharmaceutical industries.
• The proposed biosensor platforms hold potential for broader diagnostic applications.