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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
A novel electrosynthesized polymer applied to molecular imprinting technology
Ji-Lai Gong1, Fu-Chun Gong, Ge-Ming Zeng
1State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
Talanta
|October 31, 2008
Summary
Researchers developed a cholesterol sensor using molecular imprinting. This novel sensor shows good selectivity and stability for detecting electrochemically inactive analytes.
Area of Science:
- Electrochemistry
- Materials Science
- Biomedical Engineering
Background:
- Electrochemical sensors offer sensitive detection methods.
- Molecular imprinting technology enables selective analyte recognition.
- Cholesterol detection is crucial for diagnosing various health conditions.
Purpose of the Study:
- To develop a cholesterol-selective sensor using poly(2-mercaptobenzimidazole) (PMBI) films.
- To utilize molecular imprinting technology for enhanced sensor selectivity.
- To demonstrate the feasibility of detecting electrochemically inactive analytes.
Main Methods:
- Electropolymerization of PMBI films on a gold electrode surface.
- Employing molecular imprinting with cholesterol as the template.
- Utilizing differential pulse voltammetry (DPV) with ferricyanide as a mediator.
Main Results:
- The sensor demonstrated cholesterol detection up to 100 microM.
- A linear determination range was observed up to 20 microM.
- A low detection limit of 0.7 microM was achieved.
- The molecular imprinting approach provided good selectivity against coexisting substances.
Conclusions:
- The developed PMBI-based sensor exhibits high selectivity and sensitivity for cholesterol.
- Molecular imprinting is a viable strategy for creating sensors for electrochemically inactive analytes.
- The sensor is simple to prepare and offers satisfactory electrode stability.
