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An initial-rate potentiometric method for the determination of uric acid using a fluoride ion-selective electrode
Farzad Deyhimi1, Rahman Salamat-Ahangari
1Department of Chemistry, Shahid Beheshti University, Evin-Tehran, Iran. f-deyhimi@cc.sbu.ac.ir
Talanta
|October 31, 2008
Summary
A new potentiometric method uses the Emerson-Trinder reaction to detect hydrogen peroxide systems. This approach measures fluoride ions for sensitive and accurate analysis in samples like human serum.
Area of Science:
- Analytical Chemistry
- Electrochemistry
- Biomedical Analysis
Background:
- The Emerson-Trinder reaction is typically a spectrophotometric method.
- Hydrogen peroxide generating systems are common in various assays.
- A need exists for sensitive and adaptable potentiometric detection methods.
Purpose of the Study:
- To develop a general potentiometric method for assaying hydrogen peroxide generating systems.
- To adapt the Emerson-Trinder reaction for potentiometric detection.
- To demonstrate the method's utility for analyzing uric acid in biological samples.
Main Methods:
- Utilized the Emerson-Trinder reaction as a potentiometric indicator reaction.
- Detected fluoride ions released during the reaction using a fluoride ion-selective electrode.
- Employed initial-rate potentiometric measurements for quantitative analysis.
Main Results:
- Successfully applied the method to determine uric acid in aqueous and human serum samples.
- Generated initial-rate reaction progress curves and linear calibration curves.
- Reported within-day precisions, detection limits, and analytical recovery.
Conclusions:
- The developed potentiometric method offers a versatile approach for hydrogen peroxide analysis.
- The method demonstrates good performance characteristics for biological sample analysis.
- This technique expands the application of the Emerson-Trinder reaction to potentiometry.
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