Prussian blue modified glassy carbon electrodes-study on operational stability and its application as a sucrose...

Area of Science:

• Electrochemistry
• Biosensor Technology
• Materials Science

Background:

• Prussian blue (PB) films are crucial for electrochemical applications but often suffer from poor stability.
• Enhancing the stability of PB films on glassy carbon (GC) electrodes is essential for reliable biosensor development.
• Coupling PB films with oxidase enzyme systems offers potential for sensitive analyte detection.

Purpose of the Study:

• To investigate the stabilization of electrochemically deposited Prussian blue (PB) films on glassy carbon (GC) electrodes.
• To develop and demonstrate a multi-enzyme biosensor for sucrose detection utilizing stabilized PB films.
• To evaluate the performance and stability of the developed sucrose biosensor.

Main Methods:

• Electrochemical deposition of Prussian blue films on glassy carbon electrodes.
• Treatment of electrodes with tetrabutylammonium toluene-4-sulfonate (TTS) to enhance PB film stability.
• Immobilization of a tri-enzyme system (glucose oxidase, mutarotase, invertase) onto PB-modified GC electrodes using glutaraldehyde and bovine serum albumin.
• Electrochemical detection of hydrogen peroxide generated by the enzyme cascade using flow injection analysis.

Main Results:

• Tetrabutylammonium toluene-4-sulfonate (TTS) treatment significantly enhanced the operational and storage stability of PB films.
• A tri-enzyme biosensor demonstrated linear responses for sucrose (4-800 μM), glucose (2-800 μM), and H2O2 (1-800 μM).
• The biosensor achieved low detection limits (4.5 μM for sucrose) and a high sample throughput (approx. 60 samples/h).

Conclusions:

• Electrochemical activation with TTS is an effective strategy for stabilizing Prussian blue films on GC electrodes.
• The developed multi-enzyme biosensor exhibits excellent sensitivity, stability, and efficiency for sucrose determination.
• This work highlights the potential of stabilized PB films in constructing advanced electrochemical biosensing platforms.