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Updated: Jun 28, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Determination of antioxidants by solid-phase extraction method in aqueous food simulants
M S Dopico-García1, J M López-Vilariño, M V González-Rodríguez
1Laboratorio de Química, Centro de Investigacións Tecnolóxicas, Campus de Esteiro s/n, 15403 Ferrol, Spain.
Abstract:
An analytical method for the determination of specific migration levels of phenolic antioxidants and one phosphite antioxidant in the aqueous food simulants established by European legislation has been developed. This method involves solid-phase extraction (SPE) of the antioxidants with silica C(18) cartridge and their determination by liquid chromatography (LC) with diode-array detection. The achieved results in the studies of elution volume determination, breakthrough volume and accuracy are showed. Recoveries in the range of 78-104% and a relative standard deviation between 2.0 and 7.7% have been achieved.
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