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High stability amperometric biosensor based on enzyme entrapment in microgels.

Jorge Rubio-Retama¹, Enrique López-Cabarcos, Beatriz López-Ruiz

¹Departamento de Quimico Física II, Facultad de Farmacia, Universidad Complutense, Ciudad Universitaria s/n, 28040 Madrid, Spain.

|October 31, 2008

Summary

This study developed a stable amperometric glucose biosensor using glucose oxidase (GOx) immobilized in polyacrylamide microgels. The biosensor effectively measures glucose in complex samples like blood and serum, showing remarkable long-term stability.

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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

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Published on: July 11, 2012

Area of Science:

Biomedical Engineering
Materials Science
Analytical Chemistry

Background:

Amperometric biosensors are crucial for glucose monitoring.
Immobilization matrices affect enzyme stability and sensor performance.
Interference from biological sample components can compromise accuracy.

Purpose of the Study:

To develop and characterize an amperometric glucose biosensor using glucose oxidase (GOx) immobilized in polyacrylamide microgels.
To enhance sensor selectivity by mitigating interference from ascorbic and uric acid.
To investigate the impact of temperature and microgel swelling on biosensor performance and stability.

Main Methods:

Preparation of polyacrylamide microgels for GOx entrapment.
Incorporation of acrylic acid into the polymeric matrix to eliminate interference.
Characterization of the amperometric glucose biosensor's response to glucose.
Investigation of temperature effects on enzyme activity and microgel swelling.
Assessment of long-term operational and storage stability.

Main Results:

Polyacrylamide microgels serve as an effective matrix for GOx immobilization.
Inclusion of acrylic acid successfully eliminated interference from ascorbic and uric acid.
Biosensor demonstrated accurate glucose determination in complex samples (blood, serum).
Microgel swelling significantly influences enzymatic activity, with behavior mimicking free enzyme in solution.
Biosensors exhibited remarkable stability, retaining initial response after 4 months and preserved enzymatic activity for 18 months after freeze-drying.

Conclusions:

Polyacrylamide microgels offer a robust matrix for developing stable and selective amperometric glucose biosensors.
The developed biosensor is suitable for reliable glucose quantification in biological fluids.
The study highlights the importance of microgel properties and composition for optimal biosensor function and longevity.