Exploiting sequential injection analysis with lab-at-valve (LAV) approach for on-line liquid-liquid micro-extraction...

Area of Science:

• Analytical Chemistry
• Separation Science
• Miniaturized Systems

Background:

• On-line liquid-liquid extraction is crucial for sample analysis.
• Traditional methods can be time-consuming and require large solvent volumes.
• Micro-scale automation offers potential for increased efficiency and reduced waste.

Purpose of the Study:

• To develop and validate a novel on-line automated liquid-liquid micro-extraction system.
• To integrate sequential injection analysis (SIA) with a lab-at-valve (LAV) approach.
• To demonstrate the system's applicability for real-world sample analysis.

Main Methods:

• Sequential aspiration of sample, reagent, and organic solvent into a coil.
• Performing liquid-liquid micro-extraction within the coil integrated with a multiposition selection valve.
• Utilizing a conical separating chamber LAV unit for phase separation.
• Spectrophotometric monitoring of the extracted organic phase.

Main Results:

• Successful on-line automated micro-scale liquid-liquid extraction.
• Demonstrated efficacy for diphenhydramine hydrochloride (DPHH) in pharmaceutical preparations.
• Validated for the determination of anionic surfactants in water samples.

Conclusions:

• The SIA-LAV system provides a novel and efficient alternative for on-line automated micro-extraction.
• The method is suitable for the analysis of various analytes in complex matrices.
• This miniaturized approach offers advantages in terms of speed, automation, and sample/reagent consumption.