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Updated: Jun 28, 2026
Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
Published on: August 22, 2017
Interference-free determination of fluoroquinolone antibiotics in plasma by using excitation-emission matrix
Dong-Mei Fang1, Hai-Long Wu, Yu-Jie Ding
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Abstract:
Fluoroquinolones or so-called second-generation quinolones, in particular, ofloxacin (OFL), norfloxacin (NOR), and enoxacin (ENO), with therapeutic advantages possess strongly overlapped fluorescence spectra. In this paper, two strategies were proposed for simultaneous direct determination of OFL, NOR and ENO in plasma by combining fluorescence excitation-emission matrix (EEM) with second-order calibration based on the alternating trilinear decomposition algorithm (ATLD) and parallel factor analysis (PARAFAC). The results showed that both algorithms could solve the problem of serious fluorescence spectral overlapping of the sought-for analytes even in the presence of uncalibrated interferents. However, ATLD has advantages of being insensitive to overestimated component number and fast convergence. The results by using ATLD with an estimated component number of five were reasonably acceptable for clinical analysis. The average recoveries of OFL, NOR and ENO in synthetic samples were 99.7+/-2.4, 101.5+/-2.4 and 97.3+/-3.8%, respectively; the average recoveries of OFL, NOR and ENO in complex plasma were 94.3+/-2.6, 85.6+/-3.3 and 103.3+/-3.0%, respectively.
