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Published on: December 1, 2008
[Dynamic determination of sinomenine in skin by microdialysis]
Jia-Jun Ling1, Bo Xie, Yan Wang
1College of Chinese Drug, Guangzhou University of TCM, Guangzhou 510006, China. ljj@gahtcm.edu.cn
Objective:
To explore the feasibility and advantages of using microdialysis as sampling method for dynamic determination of sinomenine in topical skin.
Methods:
In this study, sinomenine was administered to the rats by transdermal drug delivery system and celiac injection. With microdialysis technique for sampling, the concentration of sinomenine in dialysate was determined by high performance liquid chromatography (HPLC).
Results:
Under existing determination condition, topical drug concentration in the skin of rats was hard to be detected after sinomenine administered to the skin, but it could be detected after celiac injection.
Conclusion:
Microdialysis sampling method can be used to determine topical drug concentration in the skin dynamically, and this method is better than traditional methods obviously.
