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Updated: Jun 28, 2026
11:40
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
Proteomics of the lysosome
Torben Lübke1, Peter Lobel, David E Sleat
1Zentrum Biochemie und Molekulare Zellbiologie, Abteilung Biochemie II, Georg-August Universität Göttingen, 37073 Göttingen, Germany.
Biochimica Et Biophysica Acta
|November 4, 2008
Summary
Lysosomal storage diseases stem from lysosome defects. New proteomic studies reveal more lysosomal proteins, aiding understanding of lysosome function and disease etiology.
Area of Science:
- Cell Biology
- Proteomics
- Human Genetics
Background:
- Lysosomal storage diseases are linked to lysosome dysfunction.
- Lysosomal proteins are increasingly implicated in complex diseases like cancer and Alzheimer's.
- Understanding lysosome composition is crucial for disease research.
Purpose of the Study:
- To explore current proteomic approaches for lysosome protein identification.
- To review newly identified lysosomal proteins.
- To highlight the implications for understanding lysosome function and disease.
Main Methods:
- Proteomic analysis using affinity purification of soluble components.
- Subcellular fractionation to isolate both soluble and membrane lysosomal proteins.
- Data interpretation of proteomic studies.
Main Results:
- Recent proteomic studies suggest a greater number of lysosomal proteins than previously known.
- Identification of novel soluble luminal and transmembrane proteins.
- These findings expand the known proteome of the mammalian lysosome.
Conclusions:
- Proteomic analysis is a powerful tool for discovering new lysosomal proteins.
- Expanded knowledge of lysosomal proteins deepens understanding of cellular functions.
- Discovery of novel proteins may uncover genetic bases for unknown human diseases.
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