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Updated: Jun 28, 2026
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Injection of dsRNA into Female A. aegypti Mosquitos
Published on: July 4, 2007
Injection of dsRNA into female A. aegypti mosquitos
Brian M Luna1, Jennifer Juhn, Anthony A A James
1Department of Molecular Biology and Biochemistry, University of California, Irvine, USA.
Journal of Visualized Experiments : Jove
|November 4, 2008
Abstract:
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.
