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Related Experiment Video

Updated: Jun 28, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

Quantitative assays for cell fusion.

Jessica H Shinn-Thomas1, Victoria L Scranton, William A Mohler

  • 1Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 4, 2008
PubMed
Summary
This summary is machine-generated.

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Accurate cell fusion assays are crucial for understanding cell biology. This study introduces a novel beta-galactosidase complementation assay for precise, high-throughput measurement of cell fusion events, including myoblast fusion.

Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Microscopic fusion index is subjective and labor-intensive.
  • Assessing cell fusion accurately is challenging in complex cellular environments.

Purpose of the Study:

  • To develop objective, high-throughput assays for cell fusion.
  • To investigate molecular factors controlling myoblast fusion.

Main Methods:

  • Developed an in vitro assay based on intracistronic complementation of beta-galactosidase activity.
  • Utilized gene knock-down protocols combined with the assay.
  • Assayed cell fusion microscopically and quantitatively in whole populations.

Main Results:

  • The beta-galactosidase complementation assay provides accurate, quantitative, and kinetic detection of cell fusion.

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A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy
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A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Published on: July 20, 2012

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

Related Experiment Videos

Last Updated: Jun 28, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy
10:45

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Published on: July 20, 2012

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
14:59

Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Published on: September 7, 2009

  • The assay is adaptable for both cell-by-cell and population-level analysis.
  • Successfully applied the assay to study factors required for myoblast fusion.

    • Conclusions:

    • Novel fusion assay enhances accuracy and throughput for cell fusion studies.
    • This method is valuable for screening molecules that regulate cell fusion.
    • Enables detailed investigation of molecular mechanisms in myoblast fusion.