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Updated: Jun 28, 2026
05:47
Isolation of Murine Lymph Node Stromal Cells
Published on: August 19, 2014
Lymph-migrating, tissue-derived dendritic cells are minor constituents within steady-state lymph nodes
Claudia Jakubzick1, Milena Bogunovic, Anthony J Bonito
1Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
The Journal of Experimental Medicine
|November 5, 2008
Summary
Lymph node dendritic cells (DCs) are not primarily derived from upstream tissues during normal conditions. Instead, migratory DCs from nonlymphoid organs mainly populate lymph nodes during inflammation.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DCs) are known to enter afferent lymphatic vessels in many organs.
- Rapid DC turnover in tissues like the lung suggests a significant migratory DC population.
Purpose of the Study:
- To investigate the origin of dendritic cells (DCs) in lymph nodes.
- To determine the contribution of migratory DCs from nonlymphoid organs to lymph node populations under homeostatic and inflammatory conditions.
Main Methods:
- Utilized the lysozyme M-Cre reporter mouse strain for tracking cell populations.
- Analyzed the relationship between lymph node DCs and nonlymphoid organ DCs.
Main Results:
- Challenged the prevailing concept that lymph node DCs are largely derived from upstream tissues during homeostasis.
- Demonstrated that nonlymphoid organ DCs become a major DC population in lymph nodes only following inflammatory stimuli.
Conclusions:
- The majority of lymph node DCs are not derived from upstream tissues under steady-state conditions.
- Inflammatory responses significantly alter the composition of lymph node DCs, leading to the infiltration of nonlymphoid organ DCs.
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