Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
What is Cell Signaling?02:03

What is Cell Signaling?

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
What is Cell Signaling?02:03

What is Cell Signaling?

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
Overview of Cell Signaling01:23

Overview of Cell Signaling

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
Overview of Cell Signaling01:23

Overview of Cell Signaling

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immersive technology applications in radiation protection for fluoroscopy - A systematic review.

Radiography (London, England : 1995)·2025
Same author

Oral health-related quality of life and factors associated with sleep bruxism in Brazilian preschool children: comparative cross-sectional study.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2025
Same author

Genitourinary symptoms and sexual function in women with primary ovarian insufficiency.

Climacteric : the journal of the International Menopause Society·2024
Same author

Functionalized bioadhesion-enhanced carboxymethyl cellulose/polyvinyl alcohol hybrid hydrogels for chronic wound dressing applications.

RSC advances·2023
Same author

Botulinum toxin in the treatment of residual limb hyperhidrosis: A systematic review.

Rehabilitacion·2023
Same author

What can anaesthetists do to help combat the global climate emergency?

Anaesthesia·2022

Related Experiment Video

Updated: Jun 28, 2026

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Nuclear calcium signaling: a cell within a cell.

M A Rodrigues1, D A Gomes, M H Nathanson

  • 1Departamento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|November 5, 2008
PubMed
Summary

Growth factors trigger nuclear calcium (Ca2+) signals via receptor tyrosine kinases, influencing cell proliferation. This nuclear signaling pathway, distinct from cytoplasmic pathways, offers new insights into cellular communication.

More Related Videos

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
08:01

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators

Published on: January 19, 2024

Related Experiment Videos

Last Updated: Jun 28, 2026

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
08:01

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators

Published on: January 19, 2024

Area of Science:

  • Cellular Biology
  • Molecular Signaling

Background:

  • Calcium (Ca2+) acts as a crucial second messenger regulating diverse cellular functions.
  • Spatial patterns of Ca2+ signals are increasingly recognized for their role in determining cellular response specificity.
  • Independent Ca2+ signaling in nuclear and cytoplasmic compartments suggests localized control, but nuclear initiation and function remain unclear.

Purpose of the Study:

  • To investigate the origin and mechanism of nuclear calcium (Ca2+) signals induced by growth factors.
  • To elucidate the role of receptor tyrosine kinases in nuclear Ca2+ signaling.
  • To explore the potential function of nuclear Ca2+ signals in growth factor-mediated cell proliferation.

Main Methods:

  • Investigated the nuclear translocation of receptor tyrosine kinases upon growth factor stimulation.
  • Analyzed the hydrolysis of phosphatidylinositol 4,5-bisphosphate and inositol 1,4,5-trisphosphate formation within the nucleus.
  • Correlated nuclear Ca2+ signaling events with cellular proliferation responses.

Main Results:

  • Receptor tyrosine kinases rapidly translocate to the nucleus following growth factor stimulation.
  • Growth factor-induced Ca2+ signals originate from nuclear phosphatidylinositol 4,5-bisphosphate hydrolysis and inositol 1,4,5-trisphosphate production.
  • Nuclear Ca2+ signaling is demonstrated to be distinct from cytoplasmic signaling pathways.

Conclusions:

  • A novel mechanism for growth factor-induced nuclear Ca2+ signaling is identified.
  • Nuclear Ca2+ signals, initiated by receptor tyrosine kinases, play a role in cell proliferation.
  • This finding provides a new paradigm for understanding how spatial Ca2+ patterns regulate cellular functions.