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Updated: Jun 28, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
Published on: June 19, 2010
Structural analysis of protein-RNA interactions with mass spectrometry
Mamuka Kvaratskhelia1, Stuart F J Le Grice
1The Ohio State University, Columbus, Ohio, USA.
Abstract:
We present a high-resolution mass spectrometric footprinting approach enabling the identification of amino acids in the protein of interest interacting with cognate RNA. This approach is particularly attractive for studying large nucleoprotein complexes that are less amenable to crystallographic or nuclear magnetic resonance analysis. Importantly, our methodology allows examination of protein-RNA interactions under biologically relevant conditions using limited amounts of protein and nucleic acid samples.
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