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Expression of epidermal growth factor mRNA in the developing mouse mandibular process.
J E Kronmiller1, W B Upholt, E J Kollar
1Department of Biostructure and Function, School of Dental Medicine, University of Connecticut Health Center, Farmington 06032.
Archives of Oral Biology
|January 1, 1991
Summary
Epidermal growth factor (EGF) mRNA is present in early mouse mandibular development but disappears by day 11. This gene expression occurs in both the epithelium and mesenchyme during critical embryonic stages.
Area of Science:
- Developmental biology
- Molecular biology
- Genetics
Background:
- The mandibular arch is crucial for craniofacial development.
- Epidermal growth factor (EGF) plays a role in embryonic development.
- Understanding gene expression patterns is key to developmental processes.
Purpose of the Study:
- To investigate the temporal and spatial expression of Epidermal Growth Factor (EGF) messenger RNA (mRNA) in the developing mouse mandibular arch.
- To determine if EGF mRNA is present in specific embryonic tissues within the mandible.
Main Methods:
- Total RNA was extracted from mouse mandibular arches at embryonic days 9 through 17.
- Reverse transcription was performed to convert RNA to complementary DNA (cDNA).
- Polymerase chain reaction (PCR) was used to amplify specific cDNA sequences, quantifying EGF mRNA expression.
Main Results:
- EGF mRNA was detected in the mandibular arch on embryonic days 9 and 10.
- EGF mRNA expression was absent in the mandibular arch from embryonic day 11 to day 17.
- Analysis of day 10 RNA preparations showed EGF mRNA present in both the epithelial and mesenchymal components of the mandible.
Conclusions:
- EGF mRNA expression is transient during early mouse mandibular development.
- EGF is likely involved in the initial stages of mandibular arch formation.
- The expression of EGF mRNA in both epithelium and mesenchyme suggests a coordinated role in early mandibular development.