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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA Base Pairing02:27

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
DNA Base Pairing02:27

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
The DNA Helix01:16

The DNA Helix

Overview

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Related Experiment Video

Updated: Jun 28, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

Electron transfer through a stable phenanthrenyl pair in DNA.

Nikolay A Grigorenko1, Christian J Leumann

  • 1Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, Bern, Switzerland.

Chemical Communications (Cambridge, England)
|November 6, 2008
PubMed
Summary

Excited pyrene-containing nucleosides facilitate electron transfer within DNA. This study observed excess electron transfer through stacked phenanthrenyl pairs to a bromouridine acceptor.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Photochemistry

Background:

  • Oligonucleotide duplexes are fundamental to genetic processes.
  • Electron transfer in DNA is crucial for biological functions and potential applications.
  • Modified nucleosides offer unique photophysical and electronic properties.

Purpose of the Study:

  • To investigate photoinduced electron transfer within a modified oligonucleotide duplex.
  • To explore the role of stacked phenanthrenyl pairs as an electron transfer mediator.
  • To determine the efficiency of electron transfer from an excited nucleoside to an acceptor.

Main Methods:

  • Synthesis of a modified oligonucleotide duplex containing 5-(pyren-1-yl)uridine and 5-bromouridine.
  • Steady-state and time-resolved spectroscopic techniques to monitor electron transfer.

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Single-Molecule F&ouml;rster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
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Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

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Single-Molecule F&ouml;rster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
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Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

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  • Computational modeling to understand the electronic interactions within the duplex.

    • Main Results:

    • Observation of efficient excess electron transfer from excited 5-(pyren-1-yl)uridine.
    • Evidence of electron transfer mediation through an internal stacked phenanthrenyl pair.
    • Quantification of electron transfer rates and yields to 5-bromouridine.

    Conclusions:

    • Stacked phenanthrenyl pairs can effectively mediate photoinduced electron transfer in oligonucleotide duplexes.
    • The study demonstrates the potential of modified nucleosides for developing DNA-based electronic systems.
    • Understanding electron transfer pathways in DNA provides insights into DNA damage and repair mechanisms.