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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

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Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

Stella Diafa1, Damien Evéquoz1, Christian J Leumann1

  • 1Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|April 25, 2019
PubMed
Summary

This study presents a protocol for synthesizing modified nucleoside triphosphates (dN*TPs) for enzymatic DNA synthesis. This method offers an alternative to traditional oligonucleotide synthesis, enabling applications like aptamer selection.

Keywords:
Bicyclo-DNADNA polymerasesNucleoside triphosphatePrimer extension reactionsSolid-phase synthesisXNA

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