TP53 gene mutations in canine osteosarcoma
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Published on: December 30, 2025
Jolle Kirpensteijn1, Marja Kik, Erik Teske
1Department of Clinical Sciences of Companion Animals and Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. j.kirpensteijn@uu.nl
Objective:
To investigate mutations of the TP53 gene in canine osteosarcoma (OS).
Study Design:
Clinical historic cohort study.
Animals:
Client-owned dogs.
Methods:
OS (n=59) were screened for mutations of the complete TP53 gene using polymerase chain reaction and the mutation was analyzed by single-strand conformational polymorphism. Clinical outcome of dogs with TP53-mutated OS were compared with dogs with OS without a mutation after complete surgical excision of the primary tumor.
Results:
TP53 gene mutations were observed in 24 of 59 (40.7%) OS; 3 mutated OS had 2 mutations. The alterations consisted mainly of point mutations (74%). Dogs with mutated OS had a significantly shorter survival time (ST) after surgery than dogs with normal tumor TP53 gene expression (P=.03). Other significant prognosticators for ST and disease-free interval included elevated serum alkaline phosphatase (P<.01) and tumor grade (P=.01).
Conclusion:
TP53 genetic mutations are common in canine OS and may have a prognostic value.
Clinical Relevance:
Mutations of the TP53 gene may influence survival and should be considered when evaluating canine OS.
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