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Related Concept Videos

Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...

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Related Experiment Video

Updated: Jun 28, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
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DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

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DOOR: a database for prokaryotic operons.

Fenglou Mao1, Phuongan Dam, Jacky Chou

  • 1Computational Systems Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.

Nucleic Acids Research
|November 8, 2008
PubMed
Summary
This summary is machine-generated.

The DOOR database offers computationally predicted prokaryotic operons from 675 genomes. This resource aids biologists in exploring bacterial and archaeal gene regulation and operon structures.

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Area of Science:

  • Microbiology
  • Bioinformatics
  • Genomics

Background:

  • Operons are fundamental units of gene organization and regulation in prokaryotes.
  • Accurate identification of operons is crucial for understanding gene expression and metabolic pathways.
  • Existing operon prediction tools vary in accuracy and scope.

Purpose of the Study:

  • To create a comprehensive database of computationally predicted prokaryotic operons.
  • To provide advanced search and analysis functionalities for operon data.
  • To serve as a centralized resource for researchers studying prokaryotic genomes.

Main Methods:

  • Development of a novel operon prediction program, validated as the top performer among 14 independent tools.
  • Compilation of predicted operons from 675 sequenced prokaryotic genomes.
  • Implementation of search capabilities including direct querying, similarity searches, and cis-regulatory motif identification.

Main Results:

  • The DOOR database houses predicted operons for 675 prokaryotic genomes.
  • The database incorporates operons for RNA genes.
  • Features include querying, similarity searches, motif identification, and an integrated wiki (OperonWiki).

Conclusions:

  • DOOR provides a valuable and accessible resource for biologists studying bacteria and archaea.
  • The database facilitates research on prokaryotic operons, gene regulation, and genome organization.
  • Advanced search functionalities enhance the utility of the operon predictions.