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Updated: Jun 28, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Adaptation of hybridomas to protein-free media results in a simplified two-step immunoglobulin M purification process

Jeremy Lee1, Anne Tscheliessnig, Allen Chen

  • 1Bioprocessing Technology Institute, Agency for Science, Technology and Research, 20 Biopolis Way, No. 06-01 Centros, Singapore 138668, Singapore.

Journal of Chromatography. A
|November 11, 2008
PubMed

An IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were significantly improved upon adaptation of the hybridoma to serum-free media. The process could even be simplified by omitting the initial tangential flow filtration step. Even with a two-step purification process a purity of >98% and a recovery of >60% was obtained.

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