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Related Experiment Video

Updated: Jun 28, 2026

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
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Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

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Bioluminescent indicators for Ca2+ based on split Renilla luciferase complementation in living cells.

Asami Kaihara1, Yoshio Umezawa, Tetsushi Furukawa

  • 1Department of Bio-Informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda, Tokyo 101-0062, Japan. kaihara.bip@mri.tmd.ac.jp

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|November 11, 2008
PubMed
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New bioluminescent indicators illuminate intracellular calcium (Ca2+) dynamics. These indicators utilize a split Renilla luciferase system, offering a novel way to visualize Ca2+ in living cells and in vivo.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Intracellular calcium (Ca2+) plays a critical role in numerous cellular processes.
  • Existing methods for monitoring Ca2+ dynamics have limitations in terms of temporal resolution or invasiveness.

Purpose of the Study:

  • To develop and characterize novel genetically encoded bioluminescent indicators for real-time monitoring of intracellular Ca2+.
  • To leverage the CaM-M13 interaction and split Renilla luciferase complementation for Ca2+ sensing.

Main Methods:

  • Construction of a genetically encoded biosensor based on split Renilla luciferase and the CaM-M13 binding pair.
  • Validation of the biosensor's Ca2+-dependent luminescence in vitro and in living cells.
  • Demonstration of the indicator's utility for imaging Ca2+ transients in response to physiological stimuli.

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Last Updated: Jun 28, 2026

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

In vivo Dual Substrate Bioluminescent Imaging
07:33

In vivo Dual Substrate Bioluminescent Imaging

Published on: October 11, 2011

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

Published on: May 23, 2014

Main Results:

  • The developed indicator exhibits Ca2+-dependent complementation of split Renilla luciferase, leading to bioluminescence emission.
  • The biosensor demonstrates high sensitivity and specificity for intracellular Ca2+.
  • Spontaneous and simultaneous bioluminescence emission allows for real-time tracking of Ca2+ dynamics.

Conclusions:

  • Genetically encoded bioluminescent indicators based on split Renilla luciferase and CaM-M13 interaction provide a powerful tool for studying Ca2+ signaling.
  • These indicators enable visualization of Ca2+ dynamics in living cells and hold potential for in vivo applications.