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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jun 28, 2026

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
09:05

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

Planar patch clamp: advances in electrophysiology.

Andrea Brüggemann1, Cecilia Farre, Claudia Haarmann

  • 1Nanion Technologies GmbH, Munich, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2008
PubMed
Summary
This summary is machine-generated.

New automated patch clamp platforms with planar chip design enable higher throughput drug screening for ion channels. Internal perfusion allows for advanced functional testing on cell membranes and artificial lipid bilayers.

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Related Experiment Videos

Last Updated: Jun 28, 2026

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
09:05

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

Whole-cell Patch-clamp Recordings in Brain Slices
07:23

Whole-cell Patch-clamp Recordings in Brain Slices

Published on: June 15, 2016

One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Area of Science:

  • Biophysics
  • Pharmacology
  • Molecular Biology

Background:

  • Ion channels are increasingly recognized as crucial therapeutic targets for various diseases.
  • Potassium channels represent the largest and most diverse ion channel family.
  • Current drug development for ion channels faces bottlenecks in functional testing.

Purpose of the Study:

  • To explore the application of internal perfusion in planar patch clamp techniques.
  • To demonstrate the utility of internal perfusion for drug screening on ion channels.
  • To investigate ion channel function in both cellular and artificial membrane systems.

Main Methods:

  • Utilized new generations of automated patch clamp screening platforms with planar chip design.
  • Applied internal perfusion during whole-cell configuration patch clamp experiments.
  • Tested potassium channels expressed in Jurkat cells and Gramicidin channels in lipid bilayers.

Main Results:

  • Planar patch clamp platforms significantly increase throughput for ion channel drug testing.
  • Internal perfusion enables simultaneous manipulation of intracellular and extracellular environments.
  • Successful application of internal perfusion demonstrated for both cell-based and artificial membrane systems.

Conclusions:

  • Automated planar patch clamp with internal perfusion overcomes previous drug screening limitations.
  • This technique expands the scope of ion channel drug discovery and research.
  • Internal perfusion is a versatile tool for studying ion channel function in diverse biological and artificial systems.