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Updated: Jun 28, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Nanoparticle-supported multi-enzyme biocatalysis with in situ cofactor regeneration
Wenfang Liu1, Songping Zhang, Ping Wang
1National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.
Abstract:
Although there have been a long history of studying and using immobilized enzymes, little has been reported regarding the nature of immobilized cofactors. Herein we report that cofactor NAD(H) covalently attached to silica nanoparticles successfully coordinated with particle-immobilized enzymes and enabled multistep biotransformations. Specifically, silica nanoparticle-attached glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH) and NAD(H) were prepared and applied to catalyze the coupled reactions for production of alpha-ketoglutarate and lactate with the cofactor regenerated within the reaction cycle. It appeared that particle-particle collision driven by Brownian motion of the nanoparticles provided effective interactions among the catalytic components, and thus realized a dynamic shuttling of the particle-supported cofactor between the two enzymes to keep the reaction cycles continuing. Total turnover numbers (TTNs) as high as 20,000h(-1) were observed for the cofactor. It appeared to us that the use of particle-attached cofactor promises a new biochemical processing strategy for cofactor-dependent biotransformations.
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