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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Published on: April 22, 2021

Structure and function of the Pre-mRNA splicing machine.

Joseph Sperling1, Maia Azubel, Ruth Sperling

  • 1Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.

Structure (London, England : 1993)
|November 13, 2008
PubMed
Summary
This summary is machine-generated.

This review details the structural mechanisms of the pre-messenger RNA (pre-mRNA) splicing machine, focusing on spliceosomes and supraspliceosomes. Understanding these structures is crucial for insights into gene regulation in development and cancer.

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Last Updated: Jun 28, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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08:53

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Genetics

Background:

  • Pre-messenger RNA (pre-mRNA) splicing removes non-coding introns from precursor transcripts.
  • Accurate splicing is essential for gene expression, development, and is frequently dysregulated in cancer.
  • Splicing occurs within a large ribonucleoprotein complex called the spliceosome.

Purpose of the Study:

  • To review the structural aspects of the pre-mRNA splicing machine.
  • To elucidate the mechanistic implications of these structures on splicing multi-intronic pre-mRNAs.
  • To provide a consistent picture by comparing in vitro spliceosome assembly with in vivo supraspliceosomes.

Main Methods:

  • Comparative analysis of structural data from various studies.
  • Integration of information on spliceosomes, their intermediates, and supraspliceosomes.
  • Focus on structural biology and mechanistic insights.

Main Results:

  • Recent progress in functional and structural analyses of the splicing machinery and its components.
  • Detailed structural information on spliceosomes and their intermediates during stepwise assembly.
  • Characterization of preformed supraspliceosomes isolated from cell nuclei.

Conclusions:

  • Structural insights are key to understanding the dynamic RNA-RNA, RNA-protein, and protein-protein interactions in splicing.
  • A comprehensive structural view aids in deciphering the mechanisms of multi-intronic pre-mRNA splicing.
  • This review consolidates structural knowledge to illuminate the splicing process.