[Molecular approach to pathology of and immunity against dermatophytes]

Molecular biological studies of the host invasion mechanisms and possible virulence-related factors of dermatophytes have just begun. The identification of individual genes and large-scale investigations of transcripts expressed under different experimental culture conditions have provided useful information on the structure, expression, and regulation of the genes of major dermatophyte species such as Trichophyton rubrum. The next goal of dermatophytosis research will be to elucidate the functions and roles of the identified fungal genes during the infection process. It will also be necessary to investigate the host immune responses to fungal gene expression and regulation during infection. For such research, genetic manipulation techniques for dermatophytes, such as exogenous gene transfer into fungal cells and targeted gene disruption, are indispensable. However, such methods are not yet well established. We have developed an efficient dermatophyte genetic manipulation system using T. mentagrophytes. Here, we present our current research findings, mainly with regard to the system for exogenous gene transfer into T. mentagrophytes cells.