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Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

Transfection of insect cell lines using polyethylenimine.

I D Ogay¹, O A Lihoradova, Sh S Azimova

¹Institute of Genetics and Plant Experimental Biology, Uzbek Academy of Science Tashkent region, Qibray district, Yuqori-Yuz, 702151, Uzbekistan.

|November 13, 2008

Summary

A novel polyethylenimine-based reagent enhances DNA transfection in insect cell lines, achieving high efficiency and viability for applications like recombinant protein production.

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Area of Science:

Molecular Biology
Biotechnology
Insect Cell Culture

Background:

Insect cell lines are crucial for recombinant baculovirus expression systems and transient gene expression.
Traditional liposome-based transfection methods are often labor-intensive and cytotoxic.
Efficient foreign DNA delivery is critical for these insect cell applications.

Purpose of the Study:

To optimize a new polyethylenimine-based DNA transfection reagent for insect cell lines.
To evaluate the efficiency and viability of this novel reagent in Spodoptera frugiperda Sf9 cells.
To assess the applicability of the developed protocol across multiple lepidopteran cell lines.

Main Methods:

Optimization of a polyethylenimine-based DNA transfection reagent.

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Last Updated: Jun 28, 2026

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)

Published on: September 13, 2012

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Published on: February 22, 2018

Delivery of plasmid vectors expressing green fluorescent protein (GFP) and GUS into insect cells.

Assessment of transfection efficiency and cell viability in Sf9 and other insect cell lines.

Main Results:

Achieved 54% transfection efficiency and 85-90% cell viability in Sf9 cells.
Successfully transfected eight different insect cell lines (Bombyx mori, Trichoplusia ni, Helicoverpa zea, Heliothis virescens, S. frugiperda) with at least 45% efficiency.
Demonstrated high heterologous protein expression levels.

Conclusions:

The developed polyethylenimine-based reagent offers an effective and viable method for DNA transfection in insect cell lines.
This protocol is suitable for transient gene expression studies in various lepidopteran cell lines.
The method supports high transfection efficacy and cell viability, improving recombinant protein expression.