Related Experiment Video
Updated: Jun 28, 2026
06:14
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
Published on: February 17, 2023
Establishment of Periodontal Ligament Cell Lines from Temperature-Sensitive Simian Virus 40 Large T-antigen
Mamoru Kubota1, Mirei Chiba, Masuo Obinata
1, , mamoru@mail.tains.tohoku.ac.jp.
Cytotechnology
|November 13, 2008
Summary
Researchers created new cell lines from periodontal ligament (PDL) cells to study how orthodontic forces cause tooth movement. These immortalized PDL cells show potential for differentiation into bone and cementum cells.
Area of Science:
- Biotechnology and Regenerative Medicine
- Cell Biology
- Orthodontics
Background:
- Orthodontic tooth movement involves cell differentiation within the periodontal ligament (PDL).
- The precise mechanisms of mesenchymal cell differentiation into osteoblasts and cementoblasts under mechanical stress are not fully understood.
- Existing in vitro systems are insufficient for studying these differentiation processes.
Purpose of the Study:
- To establish and characterize immortalized PDL cell lines for in vitro research.
- To utilize a transgenic rat model (TG rats) harboring a temperature-sensitive simian virus 40 T-antigen gene.
Main Methods:
- Periodontal ligament cells were isolated from molar roots of TG rats.
- Cells were cultured, passaged, and cloned based on colony morphology.
- Gene expression analysis (RT-PCR) and assessment of mineralized nodule formation were performed.
Main Results:
- Fifteen stable, immortalized PDL cell lines were successfully established and characterized.
- Cell lines exhibited differential gene expression patterns at 33°C, categorized into six groups.
- Three cell clones demonstrated the capacity for forming calcified nodules in vitro.
Conclusions:
- The established PDL cell lines display differential gene expression.
- Some cell lines possess the potential to differentiate into osteoblasts and cementoblasts.
- These cell lines can also express molecules involved in regulating osteoclast differentiation, crucial for orthodontic research.
