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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Updated: Jun 28, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Improved desorption electrospray ionization mass spectrometry performance using edge sampling and a rotational sample

Vilmos Kertesz1, Gary J Van Berkel

  • 1Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA. kerteszv@ornl.gov

Rapid Communications in Mass Spectrometry : RCM
|November 13, 2008
PubMed
Summary

Optimizing the sampling geometry in desorption electrospray ionization mass spectrometry (DESI-MS) significantly enhances signal intensity. A new rotational stage enables efficient

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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

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Last Updated: Jun 28, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Surface Science

Background:

  • Signal intensity in DESI-MS is highly sensitive to the relative positioning of the sample surface and the sampling capillary.
  • Previous methods often resulted in suboptimal signal levels due to inconsistent sampling geometries.

Purpose of the Study:

  • To investigate the impact of sampling geometry on DESI-MS signal intensity.
  • To develop an improved DESI-MS setup for enhanced sample analysis and throughput.

Main Methods:

  • Integration of a rotational sample stage into a standard DESI-MS system.
  • Comparison of signal intensities between 'edge sampling' and surface-touching configurations.
  • Quantitative analysis of caffeine in sport drinks using an isotopically labeled internal standard.

Main Results:

  • 'Edge sampling' geometry, where the capillary is positioned beneath the surface plane, yielded signal intensities up to five times greater than surface-touching methods.
  • The rotational sample stage facilitated efficient analysis using the optimal 'edge sampling' configuration.
  • Rapid and accurate quantification of caffeine was achieved, demonstrating the method's utility.

Conclusions:

  • Optimized 'edge sampling' geometry is crucial for maximizing DESI-MS signal intensity.
  • The developed rotational sample stage enhances the efficiency and throughput of DESI-MS analysis.
  • This approach offers a rapid and reliable method for quantitative analysis of analytes on planar surfaces.