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Related Experiment Video

Updated: Jun 28, 2026

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo
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Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo

Published on: February 17, 2019

microRNA-138 modulates cardiac patterning during embryonic development.

Sarah U Morton1, Paul J Scherz, Kimberly R Cordes

  • 1Gladstone Institute of Cardiovascular Disease, Department of Pediatrics, University of California, San Francisco, CA 94158, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 14, 2008
PubMed
Summary
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MicroRNAs (miRNAs) like miR-138 are crucial for zebrafish heart development. This study shows miR-138 establishes chamber-specific gene expression by repressing key developmental pathways.

Area of Science:

  • Developmental biology
  • Molecular biology
  • Genetics

Background:

  • Organ patterning relies on precise protein activity regulation during embryonic development.
  • MicroRNAs (miRNAs) are vital for development, but their specific roles in organogenesis are not fully understood.

Purpose of the Study:

  • To investigate the function of miR-138 in zebrafish cardiac development.
  • To understand how miR-138 regulates chamber-specific gene expression patterns.

Main Methods:

  • Utilized zebrafish as a model organism.
  • Employed antagomiRs for temporal-specific knockdown of miR-138 function during a defined developmental window (24-34 hpf).
  • Analyzed gene expression patterns and cardiomyocyte morphology.

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Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
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Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

Related Experiment Videos

Last Updated: Jun 28, 2026

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo
08:52

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo

Published on: February 17, 2019

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

Main Results:

  • miR-138 is expressed in specific zebrafish heart domains and is essential for chamber-specific gene expression.
  • Disruption of miR-138 led to ventricular expansion of gene expression and impaired ventricular cardiomyocyte morphology and cardiac function.
  • miR-138 represses aldehyde dehydrogenase-1a2 (involved in retinoic acid synthesis) and versican (cspg2) in the ventricle.

Conclusions:

  • miR-138 establishes distinct gene expression domains during cardiac morphogenesis by targeting multiple components of a shared pathway.
  • This study validates the use of antagomiRs in zebrafish for temporal miRNA knockdown studies.