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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...

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Related Experiment Video

Updated: Jun 28, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Functional characterization of alternatively spliced human SECISBP2 transcript variants.

Laura V Papp1, Junning Wang, Derek Kennedy

  • 1Signal Transduction Laboratory, Queensland Institute of Medical Research, Herston, Queensland, Australia. Laurap@qimr.edu.au

Nucleic Acids Research
|November 14, 2008
PubMed
Summary

This study reveals complex alternative splicing in the SECISBP2 gene, impacting selenoprotein synthesis and thyroid hormone metabolism. Researchers identified a novel mitochondrial isoform and demonstrated its regulation, expanding the known functions of this essential selenium metabolism protein.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Selenoprotein synthesis relies on UGA codon reassignment to selenocysteine (Sec), mediated by SECIS elements and SBP2 protein.
  • Mutations in SECISBP2 are linked to thyroid hormone metabolism abnormalities.
  • Alternative splicing of SECISBP2 has not been functionally characterized previously.

Purpose of the Study:

  • To investigate the in silico and in vivo functional consequences of alternative splicing in the human SECISBP2 gene.
  • To characterize novel SECISBP2 isoforms and their cellular localization.
  • To explore the regulatory mechanisms of SECISBP2 splicing and expression.

Main Methods:

  • In silico analysis of SECISBP2 splicing patterns.
  • Minigene-based in vivo splicing assays.
  • Antisense oligonucleotide modulation of splicing.
  • Analysis of transcriptional and translational regulation under stress conditions.

Main Results:

  • Identified a complex splicing pattern in the 5'-region of SECISBP2, generating at least eight splice variants encoding five isoforms.
  • Discovered a novel isoform, mtSBP2, with a mitochondrial targeting sequence and demonstrated its mitochondrial localization.
  • Showcased that mtSBP2 splicing can be modulated by antisense oligonucleotides.
  • Revealed coordinated transcriptional and translational regulation of SBP2 variants under UVA irradiation stress.

Conclusions:

  • Alternative splicing significantly diversifies SECISBP2 function and protein isoforms.
  • The mtSBP2 isoform represents a new player in mitochondrial function or regulation.
  • SECISBP2 expression is dynamically regulated by splicing and stress responses, extending its role beyond basic selenium metabolism.