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Updated: Jun 28, 2026
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Published on: August 28, 2017
Enzymes containing porous polymersomes as nano reaction vessels for cascade reactions
Suzanne M Kuiper1, Madhavan Nallani, Dennis M Vriezema
1Molecular Materials, Institute for Molecules and Materials, University of Nijmegen, Toernooiveld 1, Nijmegen, Gelderland, Netherlands 6500 GL.
Abstract:
Polystyrene(40)-b-poly(isocyanoalanine(2-thiophen-3-yl-ethyl)amide)(50) (PS-PIAT) polymersomes have the unique property of being sufficiently porous to allow diffusion of small (organic) substrates while retaining large biomolecules such as enzymes inside. Herein we report on the encapsulation and protection of glucose oxidase (GOx) and horse radish peroxidase (HRP) in PS-PIAT polymersomes and the successful employment of these functionalised nanoreactors in a cascade reaction. The demonstrated concept allows for further application in other enzymatic cascade reactions, bio-organic hybrid systems and biosensing devices.
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