Related Experiment Video
Updated: Jun 28, 2026

Separation of Aldehydes and Reactive Ketones from Mixtures Using a Bisulfite Extraction Protocol
Published on: April 2, 2018
Separation and quantitative analysis of alkyl sulfate ethoxymers by HPLC
Julien Morvan1, Marie Hubert-Roux, Valérie Agasse
1Laboratoire Synthèse et Chromatographie, Sciences et Méthodes Séparatives, UPRES EA 3233, Université de Rouen, rue Tesnières, F-76821 Mont Saint Aignan Cedex, France.
Abstract:
Separation of alkyl sulfate ethoxymers is investigated on various high-performance liquid chromatography (HPLC) stationary phases: Acclaim C18 Surfactant, Surfactant C8, and Hypercarb. For a fixed alkyl chain length, ethoxymers are eluted in the order of increasing number of ethoxylated units on Acclaim C18 Surfactant, whereas a reversed elution order is observed on Surfactant C8 and Hypercarb. Moreover, on an Acclaim C18 Surfactant column, non-ethoxylated compounds are eluted in their ethoxymers distribution and the use of sodium acetate additive in mobile phase leads to a co-elution of ethoxymers. HPLC stationary phases dedicated to surfactants analysis are evaluated by means of the Tanaka test. Surfactant C8 presents a great silanol activity whereas Acclaim C18 Surfactant shows a high steric selectivity. For alkyl sulfates, linearity of the calibration curve and limits of detection and quantitation are evaluated. The amount of sodium laureth sulfate raw material found in commercial body product is in agreement with the specification of the manufacturer.
More Related Videos
09:47Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers
Published on: November 22, 2015
11:44Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
Published on: March 6, 2016
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Elution Process
Analyte Adsorption and Distribution