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Updated: Jun 28, 2026

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
Published on: October 11, 2022
A novel fluorescent in situ hybridization technique for detection of Rickettsia spp. in archival samples
Claus Bo Svendsen1, Mette Boye, Carsten Struve
1Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark. clv@ssi.dk <clv@ssi.dk>
Abstract:
A novel, sensitive and specific method for detecting Rickettsia spp. in archival samples is described. The method involves the use of fluorescently marked oligonucleotide probes for in situ hybridization. Specific hybridization of Rickettsia was found without problems of cross-reactions with bacterial species shown to cross-react serologically.
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