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The Eukaryotic Promoter Region

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

The Eukaryotic Promoter Region

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Related Experiment Video

Updated: Jun 28, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Complex assembly on the human CYP17 promoter.

Marion B Sewer¹, Srinath Jagarlapudi

¹School of Biology and the Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, United States. marion.sewer@biology.gatech.edu

Molecular and Cellular Endocrinology

|November 15, 2008

Summary

Adrenocorticotropin (ACTH) regulates steroid hormone production by coordinating gene transcription in the adrenal cortex. This involves protein complexes binding to gene promoters, influencing steroidogenesis.

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Area of Science:

Endocrinology
Molecular Biology
Gene Regulation

Background:

Steroid hormone biosynthesis relies on coordinated gene transcription.
Adrenocorticotropin (ACTH) in the human adrenal cortex initiates a signaling cascade for steroidogenesis.

Purpose of the Study:

To elucidate the mechanisms of CYP17 transcription regulation.
To understand the role of transcription factors and coregulatory complexes in steroidogenesis.

Main Methods:

Analysis of transcription factor recruitment to the CYP17 promoter.
Investigation of higher-order coregulatory complex formation.
Examination of enzymatic modifications of transcription factors and histones.

Main Results:

Multiple transcription factors (SF-1, GATA-6, SREBP1) are recruited to the CYP17 promoter.
Formation of higher-order coregulatory complexes is essential for increased CYP17 mRNA expression.
Enzymatic activities within these complexes post-translationally modify transcription factors and histones.

Conclusions:

Transcription factors and coregulatory proteins dynamically regulate CYP17 transcription.
Kinases, phosphatases, acetyltransferases, and histone deacetylases are key regulators of CYP17 mRNA expression.
Understanding these mechanisms is crucial for steroidogenesis research.