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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Eukaryotic Compartmentalization01:37

Eukaryotic Compartmentalization

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
Eukaryotic Compartmentalizations01:46

Eukaryotic Compartmentalizations

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
Eukaryotic Compartmentalization01:46

Eukaryotic Compartmentalization

One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: Jun 28, 2026

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Towards eukaryotic structural complexomics.

Christoph Bieniossek1, Imre Berger

  • 1Institute for Molecular Biology and Biophysics, ETH Hönggerberg, 8093, Zurich, Switzerland.

Journal of Structural and Functional Genomics
|November 15, 2008
PubMed
Summary
This summary is machine-generated.

We developed a rapid and flexible method for expressing complex protein assemblies using multigene baculoviral vectors. This strategy streamlines structural biology research and is adaptable for automation in various expression hosts.

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Area of Science:

  • Structural molecular biology
  • Protein biochemistry
  • Recombinant protein expression

Background:

  • Eukaryotic proteins often function as large multisubunit assemblies.
  • Challenges exist in expressing these complexes due to size and subunit number.
  • Conventional cloning and expression methods are labor-intensive and lack flexibility.

Purpose of the Study:

  • To develop a rapid and flexible method for multiprotein complex expression.
  • To facilitate structural biology studies requiring protein diversity.
  • To create a strategy adaptable for automation and various expression hosts.

Main Methods:

  • Utilized multigene baculoviral vectors.
  • Employed recombination-mediated assembly for complex formation.
  • Focused on baculovirus/insect cell expression systems.

Main Results:

  • Developed reagents and protocols for efficient multiprotein complex expression.
  • Demonstrated a strategy suitable for structural biology applications.
  • Showcased flexibility for introducing mutations, truncations, and purification tags.

Conclusions:

  • The developed method enables rapid and flexible expression of complex protein assemblies.
  • The strategy is adaptable for automation and can be applied to mammalian or prokaryotic hosts.
  • This approach accelerates research in structural molecular biology and protein science.