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Updated: Jun 26, 2026

09:25
Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
Published on: January 9, 2015
Correction to "Cryo-EM Structure of the FtsH Periplasmic Domain Reveals Functional Dynamics"
ACS Chemical Biology
|June 25, 2026
Summary
No abstract available in PubMed .
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Cryo-electron Microscopy
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Protein Folding
Overview

