Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Long-term Potentiation01:35

Long-term Potentiation

Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Long-term Potentiation01:25

Long-term Potentiation

Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when presynaptic neurons...
Long-term Depression01:03

Long-term Depression

Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over time, all...
Long-term Depression01:05

Long-term Depression

Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glutamatergic pathway from deep layers of the insular cortex to the rostral central medial thalamic nucleus participates in neuropathic pain and anxiety-like behaviors in mice.

The journal of headache and pain·2026
Same author

Orientia tsutsugamushi targets lamin A using Ank effectors and alters chromatin to inhibit NF-κB.

Communications biology·2026
Same author

Norepinephrinergic projection from locus coeruleus to parafascicular nucleus promotes pain and anxiety-like behaviors in mice.

JCI insight·2026
Same author

Neuronal nitric oxide synthase expression in secretory cells of the human parotid and submandibular salivary glands: evidence from light and electron microscopy.

Cell and tissue research·2026
Same author

Activating the vlPAG-LC neural pathway alleviates neuropathic pain and comorbid anxiety-like behaviors through distinct projections.

Neurobiology of disease·2026
Same author

Gamma-ray spectra and absorbed doses measured at EuXFEL undulator system.

Journal of synchrotron radiation·2025

Related Experiment Video

Updated: Jun 27, 2026

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
09:39

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

Published on: June 26, 2013

Persistent LTP without triggered protein synthesis.

Abdul-Karim Abbas1, Mikhail Dozmorov, Rui Li

  • 1Department of Medical Biophysics, Institute of Neuroscience and Physiology, Gothenburg University, Box 433, 40530 Gothenburg, Sweden. karim.abbas@physiol.gu.se

Neuroscience Research
|November 18, 2008
PubMed
Summary
This summary is machine-generated.

Long-term potentiation (LTP) in young rats does not require new protein synthesis for stabilization up to 8 hours. Existing proteins are sufficient for maintaining synaptic plasticity during this period.

More Related Videos

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
11:29

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

Published on: September 4, 2015

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
12:25

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Published on: September 28, 2018

Related Experiment Videos

Last Updated: Jun 27, 2026

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
09:39

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

Published on: June 26, 2013

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
11:29

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

Published on: September 4, 2015

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
12:25

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Published on: September 28, 2018

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cellular Physiology

Background:

  • Synaptic plasticity, crucial for learning and memory, is thought to involve protein synthesis for long-term stabilization.
  • A distinction is made between early (E) and late (L) phases of synaptic plasticity based on duration, with L-phase effects (>2-3h) traditionally linked to new protein synthesis.
  • The precise role of newly synthesized versus existing proteins in synaptic plasticity, particularly in young organisms, remains incompletely understood.

Purpose of the Study:

  • To investigate the necessity of de novo protein synthesis for the stabilization of long-term potentiation (LTP) in the CA1 region of young rat hippocampus.
  • To determine if protein synthesis inhibitors applied during a critical induction window affect the duration of LTP.
  • To clarify whether established proteins suffice for maintaining LTP in the absence of triggered protein synthesis.

Main Methods:

  • Assessment of LTP in hippocampal CA1 slices from 12- to 20-day-old rats.
  • Application of protein synthesis inhibitors anisomycin and emetine around the time of LTP induction (-30 min to +30 min).
  • Monitoring LTP stability over extended recording periods (4h for anisomycin, 8h for emetine) and verifying inhibitor efficacy through baseline response and radiolabeling assays.

Main Results:

  • Long-term potentiation remained stable for up to 4 hours with anisomycin and 8 hours with emetine, despite their application during the critical induction phase.
  • Emetine's effectiveness was confirmed by its ability to block baseline responses with prolonged exposure.
  • Anisomycin's potency was validated by its inhibition of yeast growth and confirmed by radiolabeling experiments measuring leucine incorporation, indicating effective inhibition of protein synthesis.

Conclusions:

  • Long-term potentiation in the hippocampal CA1 region of young rats can be maintained for at least 8 hours without requiring the synthesis of new proteins.
  • These findings suggest that proteins already present at the time of induction are sufficient to support the stabilization of LTP.
  • The study challenges the traditional view that late-phase LTP necessitates triggered protein synthesis, highlighting the potential role of pre-existing protein machinery.