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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jun 27, 2026

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
07:24

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples

Published on: August 31, 2018

Microarray analysis identifies candidate genes for key roles in coral development.

Lauretta C Grasso1, John Maindonald, Stephen Rudd

  • 1Centre for the Molecular Genetics of Development, Research School of Biological Sciences, Australian National University, Canberra, Australia. lauretta.grasso@anu.edu.au

BMC Genomics
|November 19, 2008
PubMed
Summary
This summary is machine-generated.

This study reveals key genes involved in coral development, including calcification and symbiont acquisition. Some genes show surprising links to higher animals, offering insights into coral biology and evolution.

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Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
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Published on: July 30, 2011

Area of Science:

  • Marine Biology
  • Developmental Biology
  • Genomics

Background:

  • Anthozoan cnidarians, like stony corals, possess a complex gene repertoire despite their simple tissue organization.
  • Stony corals are ecologically vital to tropical reef ecosystems.
  • Understanding coral development, skeletogenesis, and symbiont acquisition is crucial.

Purpose of the Study:

  • Investigate the molecular basis of cnidarian development.
  • Identify genes involved in coral-specific processes like skeletogenesis and symbiont uptake.
  • Analyze gene expression during early coral development.

Main Methods:

  • Utilized microarray analysis to examine gene expression across four developmental stages.
  • Focused on the period of skeletogenesis initiation and symbiont acquisition.
  • Employed whole-mount in situ hybridization to determine spatial gene expression patterns.

Main Results:

  • 1084 out of 5081 unique peptide coding genes were differentially expressed during coral development (P
  • Identified candidate genes related to settlement, metamorphosis, calcification, and symbiont interaction.
  • Characterized spatial expression patterns of these key developmental genes.

Conclusions:

  • This is the first large-scale study of cnidarian developmental gene expression, identifying candidate genes for crucial coral biological processes.
  • Discovered genes with counterparts in higher animals but absent in closely related sea anemones.
  • Suggests coral-specific traits may involve analogous processes to distantly related organisms, highlighting microarray's potential for coral research.