Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparison of four methods for free thyroxin.

D Deam1, M Goodwin, S Ratnaike

  • 1Biochemistry Department, Royal Melbourne Hospital, Victoria, Australia.

Clinical Chemistry
|April 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determination of the source location of anthropogenic radionuclides collected in Finland and Sweden in June 2020 using a multi-technology analysis.

Journal of environmental radioactivity·2024
Same author

Third international challenge to model the medium- to long-range transport of radioxenon to four Comprehensive Nuclear-Test-Ban Treaty monitoring stations.

Journal of environmental radioactivity·2022
Same author

Detection of subtle cartilage and bone tissue degeneration in the equine joint using polarisation-sensitive optical coherence tomography.

Osteoarthritis and cartilage·2022
Same author

Determination of the <sup>161</sup>Tb half-life.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2022
Same author

Recruitment and Consent in an observational study.

Community dental health·2020
Same author

CRISPR-based gene editing enables <i>FOXP3</i> gene repair in IPEX patient cells.

Science advances·2020
Same journal

Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

Clinical chemistry·2026
Same journal

Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

Clinical chemistry·2026
Same journal

Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

Clinical chemistry·2026
Same journal

TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

Clinical chemistry·2026
Same journal

Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

Clinical chemistry·2026
Same journal

Editor's Note: Circulating Proteolytic Products of Carboxypeptidase N for Early Detection of Breast Cancer.

Clinical chemistry·2026
See all related articles

This study evaluated four free thyroxin (FT4) assays, finding that manufacturer reference ranges may need adjustment for specific patient populations, including those with low albumin or thyroxin autoantibodies.

Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Diagnostic Assays

Background:

  • Accurate measurement of free thyroxin (FT4) is crucial for diagnosing thyroid disorders.
  • Several commercial FT4 assays exist, but their performance can vary across different patient populations and clinical conditions.

Purpose of the Study:

  • To evaluate and compare the performance of four different free thyroxin (FT4) reagent kits.
  • To assess the influence of factors like albumin levels, thyroxin autoantibodies, and pregnancy on FT4 assay results.
  • To determine if manufacturer-provided reference ranges are suitable for local laboratory use.

Main Methods:

  • A limited evaluation of four FT4 reagent kits: Amerlex-M (AFT4), Amerlite (LFT4), MagicLite (MFT4), and GammaCoat two-step RIA (GFT4).
  • FT4 levels were measured in 201 subjects with diverse clinical conditions, including healthy controls, hypothyroid/thyrotoxic patients, and those with specific interfering factors.

Related Experiment Videos

  • Analysis included correlations with serum albumin, thyroxin-binding globulin (TBG), and assessment during pregnancy.

    • Main Results:

    • No correlation was found between thyroxin-binding globulin (TBG) and FT4 across all assays.
    • Only the Amerlex-M (AFT4) assay showed a significant correlation with albumin concentrations.
    • Thyroxin autoantibodies interfered with all assays except GammaCoat (GFT4).
    • All methods indicated a decrease in mean FT4 values during late pregnancy.

    Conclusions:

    • The performance of FT4 assays can be affected by patient-specific factors such as albumin levels and autoantibodies.
    • Manufacturer reference ranges may require modification for accurate FT4 interpretation in specific patient groups and local laboratory settings.
    • The GammaCoat (GFT4) assay demonstrated better resistance to interference from thyroxin autoantibodies compared to other tested methods.